Anti-inflammatory Effect of Serum of Osteoarthritis Patients After Administration of the Oléogrape®SEED, an Extract of Grape and Olive

Osteoarthritis is a degenerative disease that affects a growing proportion of the population. There is currently no treatment to halt this process. The aim of the research is in particular to find treatments targeting the molecular origins of osteoarthritis focusing especially on the inflammatory component of the disease. That is why research is looking to the development of preventive treatments like nutraceuticals. This project aims to determine the anti-inflammatory properties of sera of patients who received supplementation of their diet with an extract of grape and olive: the Oléogrape®SEED. Sera and synovial fluid will be collected during visits for hyaluronic acid injections in OA patients. These sera will be tested in vitro in a model of inflamed chondrocytes.

Inclusion Criteria: * 50 years old and over * Patients with knee osteoarthritis (ACR criteria) * Patients requiring injection of hyaluronic acid (no corticosteroid associated) * Radiological stage of knee osteoarthritis 1, 2 or 3 (Kellgren-Lawrence) * Informed consent form signed Exclusion Criteria: * younger than 50 years * protected adult * Pregnant woman * Radiological Stage 4 osteoarthritis (Kellgren-Lawrence) * knee osteoarthritis secondary to arthritis * Taking a long term treatment of osteoarthritis (Piasclédine®, Diacéréine®, glucosamine, chondroitin) * inflammatory pathology other than osteoarthritis * Taking anti-inflammatory medications * Refusal to participate in the study * Use of food supplements with anti-inflammatory properties * Arthroscopy less than 6 months * corticosteroid injection \ 4 or TCA\> 2 (anticoagulants) * Being allergic to lactose