Discovery of Biological Signatures for Cruciferous Vegetable Intake (Single Serving)

The purpose of this project is to study the molecular and metabolic profiles in human plasma, urine and microbiome composition following the consumption of broccoli sprouts.

Inclusion Criteria: * Healthy men and women, 18-60 years of age * Willing to stop taking herbal and phytochemical (plant-based extract or phytochemical) supplements for 1 week prior to and during the study * Willing to stop cruciferous vegetable and probiotic supplement, food and beverage intake 1 week prior to and during the study * Willing to complete a 10-day food diary during the study * Must be able to give written informed consent Exclusion Criteria: * Body Mass Index (BMI) \30.0 kg/m2 * Tobacco use, including e-cigarettes, or smoking of any substance (e.g. cannabis) in the past three months * Pregnancy, breastfeeding, or planning to become pregnant before completing the study * Engaging in vigorous exercise more than 7 hours per week * Have a significant acute or chronic illness such as cardiovascular disease, kidney or liver disease, diabetes, thyroid disorder, or radiation or chemotherapy treatment for cancer within the past five years. * Use of medications to control cholesterol (e.g. statins, cholestyramine) or fat absorption (e.g. orlistat) * Have had bariatric surgery (e.g. gastric bypass, gastric banding, sleeve gastrectomy, etc.), other gastrointestinal procedure (e.g. cholecystectomy) or disorders (e.g. Crohn's disease, celiac disease, ulcerative colitis) * Use of oral antibiotic medication within the 6 months