The Human Sperm Survival Assay (HSSA) as an Internal Quality Control for the IVF Consumables (REPROTOX 2)

The main embryotoxicity quality controls are the mouse embryo test (MEA = Mouse Embryo Assay) and the human sperm survival test (HSSA = Human Sperm Survival Assay). The HSSA test measures the survival (or mobility) of human sperm after exposure to the tested consumable for a predetermined period of time and compares it to that of unexposed sperm. It would appear that the HSSA is comparable in terms of sensitivity to the MEA test for the detection of toxicity.

Inclusion Criteria: * Male over 18 years-old * Normal spermogram (concentration ≥15 million / ml or total count\> 39 million, progressive mobility ≥32% or total mobility ≥40%) * Progressive mobility ≥70% after selection * Total number of progressive motile spermatozoa recovered after TMS\> 10 million * Patient affiliated or beneficiary of a social security scheme * Patient having been informed and not opposing to this research. Exclusion Criteria: * Male over 65 years-old * Protected patient: adult under guardianship, curatorship or other legal protection, deprived of liberty by judicial or administrative decision