Jejunal Luminal and Colonic Mucosa-Associated Microbiota in Metabolic Diseases: Methods, Identification and Causalities

This clinical study will investigate Jejunal Microbiota in Metabolic Diseases (Je-MiMe, n=45) and Colonic mucosa-associated Microbiota in Metabolic Diseases (Col-MiMe, n=45). Each cohort (Je and Col -MiMe) is composed of three groups: participants with obesity (n=15) with obesity and with type II diabetes (n=15). This research protocol is organized in two parts. Each part of this study will recruit 45 participants that are only recruited in one of the parts of the protocol (JE-MIME or COL-MIME). Thus, in total, the study will include 90 patients. Each part of this study is composed of 3 groups: 1) "Control Group ", 2) Obese group (Ob), 3) Obese and Type 2 Diabetes group (ObD). Control groups for part I (JE-MIME) and part 2 (COL-MIME) are composed of different participants. Each group is composed of 15 human adult volunteers for the JE-MIME study (part 1) and 15 participants for the COL-MIME study (part 2). Total number of participants is 45 for part 1, and 45 for part 2. Total number for this project is 90. Microbiota wil be collected during an endoscopy or coloscopy which is planned as routine care for the patients. Primary objectives are to characterize jejunal (JE-MIME, Part I of the study) and colonic mucosa-associated microbiota (COLMIME, Part II of the study) and compare both microbiota to faecal microbiota (evaluate differences and similarities between jejunal microbiota or mucus-associated microbiota and faecal microbiota). Secondary objectives are to 1) Correlate microbiota with metabolic health and inflammatory markers; 2) Correlate microbiota with lifestyle and neuropsychological health. Both the jejunal microbiota and mucus-associated microbiota are strong integrators of nutritional environment and intestinal health status, respectively, compared to the fecal microbiota. This study will help to better understand the physiopathology of metabolic diseases. This research could lead to finding specific microbiota members, either from the jejunal compartment or from the inner mucus layer, crucial for the promotion / protection of chronic intestinal inflammation and associated metabolic health.

Inclusion Criteria: * General Inclusion Criteria: * Aged between 18 and 60 years; * Ability to understand and provide informed consent (in French); * Ability and willingness to meet the required schedule and study procedures; * Group-Specific Inclusion Criteria: * Control Group Inclusion criteria for the participants from the Control group are: * Presence of Gastroesophageal Reflux Disease symptoms or gastric discomfort. * BMI \[19kg/m² \< BMI \ 150 μmol/l or eGFR \< 60 ml/min per 1.73 m2 body surface area) * CVD, endocrine, renal or other chronic disease likely to affect motility. * Colon cleansing preparation during the last 1 month * No \< 3 bowel movements per week * Females of childbearing age who do not practice birth control and/or are pregnant or lactating * Participants non-affiliated to the French national health scheme * Participants who are already included in a clinical study which implies testing any pharmaceutical drug. * Participants who do not understand the research procedures those who are institutionalized, or who unable to give informed consent * Participants placed under legal protection * Patients with drug addiction * Antibiotherapy 3 months preceding the endoscopy * Weight variation (diminution or increase) \> 5kg in the last 3 months * Specific non-inclusion Criteria * Ob and ObT2D Group * Usual contraindication for bariatric surgery;