Rapid Detection Method for Microorganisms in the Lower Genital Tract

Mycoplasma, chlamydia, and gonococcal infection of the female lower genital tract can lead to infertility and various adverse pregnancy outcomes. The lower genital tract bacterial community status type (CST) is closely related to genital tract infection and reproductive health. However, there is a need for a rapid, accurate, stable, and economical detection method to detect the above pathogens and CST at the same time, so there is also a need for evaluation of the correlation between each pathogen infection and CST. Matrix assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) is more stable and accurate than traditional detection methods such as culture and staining. It can simultaneously detect a variety of microorganisms and their subtypes, while the high-throughput sequencing method is faster and more economical, which is suitable for detecting multiple microorganisms in clinical practice. The research group has established a perfect MALDI-TOF MS platform in the early stage and put it into clinical testing. This project will establish a new rapid detection method for lower genital tract microorganisms based on MALDI-TOF MS. Then, examine the lower genital tract microecology of infertile patients and healthy women using the newly developed method. At last, analyze the correlation between pathogen infection, CST status, and infertility. This project will solve the practical work needs of clinicians for a comprehensive assessment of female lower genital tract CST and common pathogen infection and fill in the technical gaps in the microbial examination of the lower genital tract.

Inclusion Criteria:Case group:1) Female aged 18-40 2) Regular menstrual cycle 2) Conform to the clinical diagnosis of infertility 3) No sexual life 3 days before the examination control group:Female aged 18-40Regular menstrual cyclePatients who did pre-pregnancy consultation in our hospital, pregnant at the time of 3-month follow-upNo sexual life 3 days before the examination -Exclusion Criteria:Case group:Infertility due to malformation of reproductive tractInfertility caused by endocrine factors such as polycystic ovary syndrome, premature ovarian failure and severe hyperthyroidismInfertility due to male factorsPatients with malignant tumorsPatients with autoimmune diseasesPatients with severe heart, kidney and other basic diseasesPatients who consumed antibiotics 3 days before the examinationHave the habit of smoking and drinkingControl group:Pregnancy by various assisted reproductive meansPatients with malignant tumorsPatients with autoimmune diseasesPatients with severe heart, kidney and other basic diseasesPatients who consumed antibiotics 3 days before the examinationHave the habit of smoking and drinking