Microscopy Imaging of Whole-mount Stained Human Tissues

The goal of this clinical trial is to develop an imaging platform for intraoperative tumor margin delineation in 250 cases of tumor-suffered patients. The main questions it aims to answer are:• to develop the protocol of rapid assessment of surgical specimens without need for fixation, embedding, and cryosectioning required for conventional histopathology.Participants will provide a small piece of their surgical specimens from tumor removal surgery .If there is a comparison group: Researchers will compare normal specimens to see if we can observe the difference.

Inclusion Criteria:Patients who are more than 20 years old.Diagnosed as patients suffered by skin tumor, brain tumor and breast tumor.Exclusion Criteria:Patients who can't speak and communicate with others.Patients who can't make eventual and exact pathological assessment for his/her diseases.Patients who only can fit for slicing tumor.Removed tissues which obviously vary after severe ray radiation under microscopy observation.Diameter of tumors are under 1 centimeter.Breast carcinoma in situ.