Lung Cancer Diagnosis by Detecting Epigenetic Imprinting Alterations in Bronchoalveolar Lavage

The goal of this multicenter observational study is to evaluate the lung cancer diagnostic value of epigenetic imprinting detection in bronchoalveolar lavage. This study will mainly focus on varifing the previously identified epigenetic imprinting biomarkers for lung cancer and upgrading and validating a lung cancer imprinting diagnostic model specifically for bronchoalveolar lavage. The lavage sample will be collected from each eligible paticipants under bronchialscopy and undergo QCIGISH detection to analyze the allelic expression status of imprinted genes. The QCIGISH detection results will be compared with the final surgical histopathology. No interventions will be taken according to the QCIGISH detection results.

Inclusion Criteria:Age ≥ 18 years old, and ≤ 75 years old;Chest CT showed pulmonary nodule with diameter<3cm;Subjects voluntarily participated and signed the informed consent form.Exclusion Criteria:Active massive hemoptysis;Severe heart and lung dysfunction;Severe arrhythmia;Extreme exhaustion of general condition;Coagulation dysfunction;Acute attack of asthma;Aortic aneurysm.