Brain Connectivity Analysis

Stroke remains the leading cause of permanent disability. The neuromodulation technique of transcranial Direct Current Stimulation (tDCS) has the potential to serve as an adjuvant therapy to enhance neuronal connectivity following stroke. By combining electroencephalography (EEG) and tDCS, this study aims to investigate changes in network connectivity and cerebral plasticity, which are key factors in functional recovery after stroke. The objectives are to: 1) analyze how tDCS modulates neuronal activity, 2) evaluate persistent effects in follow-up assessments, and 3) predict patient outcomes. Studies on human subjects will be complemented by research in a mouse model of stroke to: 1) identify the molecular mechanisms underlying tDCS effects on functional recovery, 2) establish correlations among functional, molecular, and connectivity indices, and 3) assess the efficacy of biomimetic nanoparticles in targeting injured brain tissue to reduce inflammation, enhance neuronal plasticity, and support functional recovery.

Inclusion Criteria: Age 18-90 * First ischemic stroke occurring 3-30 days after onset * Residual upper limb function * Ability to provide informed consent and understand instructions Exclusion Criteria: * Severe spasticity (Ashworth \>2) * Presence of additional neurological diseases * Significant comorbidities * History of intracerebral hemorrhage * Severe neglect * Contraindications to tDCS