Hyaluronic Acid and Albumin-PRF in Non-Surgical Periodontal Therapy

Periodontitis is a chronic inflammatory disease that leads to the destruction of the supporting tissues of the teeth. Non-surgical periodontal treatment is the first-line approach for the management of periodontitis; however, the use of adjunctive therapeutic agents may improve clinical outcomes. The aim of this randomized clinical trial is to evaluate the effects of hyaluronic acid gel and albumin platelet-rich fibrin (Alb-PRF) used as adjuncts to non-surgical periodontal treatment on clinical and biochemical parameters in patients with Stage III periodontitis. Participants will be randomly allocated into three groups: an Alb-PRF group, a hyaluronic acid gel group, and a control group receiving non-surgical periodontal treatment alone. Clinical periodontal parameters and biochemical markers obtained from gingival crevicular fluid will be assessed at baseline and at 1 and 3 months after treatment. The findings of this study are expected to contribute to the evaluation of the potential benefits of adjunctive biological and anti-inflammatory applications in the non-surgical periodontal treatment of patients with Stage III periodontitis.v

Inclusion Criteria: * Systemically healthy individuals aged between 30 and 60 years * No history of periodontal treatment or antibiotic use within the last 6 months * Non-smokers * Accepting to regularly attend the follow-up sessions planned during the study period * Diagnosis of Stage III periodontitis * Presence of at least 15 teeth in the oral cavity, with more than 30% of the existing teeth affected by periodontal disease * Presence of probing pocket depth ≥6 mm and clinical attachment loss ≥5 mm in affected teeth * Presence of vertical bone loss ≥3 mm * Radiographic evidence of alveolar bone loss extending to the middle third or beyond (≥33%) of the root length in affected teeth Exclusion Criteria: * Presence of any systemic disease * History of periodontal treatment or antibiotic use within the last 6 months * Pregnancy or lactation * Presence of acute oral lesions or necrotizing ulcerative periodontitis * Use of medications known to affect periodontal tissues * Current smoking * Presence of immune system disorders or use of immunosuppressive medications * Presence of prosthetic restorations on teeth selected for gingival crevicular fluid sampling