Functional Regulation of Autoreactive Lymphocytes: Phenotypic and Transcriptomic Investigations in Healthy Subjects

The goal of this study is to study the autoreactive lymphocytes in healthy volunteers. More specifically, this research aims to identify what distinguishes the autoreactive lymphocytes found in healthy individuals from those observed in patients, and to understand the mechanisms that prevent the onset of autoimmune disease in healthy individuals. Participants will have a single visit where they will undergo a blood drawing (56mL). Some participants will be proposed to join a longitudinal cohort consisting in two supplementary visits at M12 and M24 where they will undergo a new blood drawing (56mL).

Inclusion Criteria: * Adults aged between 18 ans 69 * Weight \> 50kg * Able to give free and informed consent * Patient from the local area (livingin the region where the recruitment centre is located) Non-inclusion Criteria: * History of cancer within the last 5 years * Autoimmune or chronic inflammatory disease * Current infection (acute or chronic) * Severe asthma * Recent heavy bleeding * Anticoagulant therapy, coagulation disorder, anaemia, cardiovascular disease (valvular heart disease, arrhythmias, arterial vascular insufficiency) * Pregnancy and the postpartum period (6 months; self-reported on questionnaires) * Poor tolerance to blood donation (whether or not related to haemodynamic instability) * History of seizures or epilepsy * History of stroke, transient ischaemic attack or repeated episodes of syncope * Immunosuppressive treatment (current oral or injectable corticosteroids, CYC, MTX/LEF, AZA/MMF, anti-CD20, etc.) * Persons deprived of their liberty * Persons receiving psychiatric care * Persons admitted to a health or social care facility * Adults subject to a legal protection order